T.P. ASSOCIATES INTERNATIONAL, INC.
17875 Cherokee P.O. Box 96
Harpster, Ohio 43323
FAX # 614-496-4121
614-496-4571

Attn: Mr. Sheldon Seaborn
American Crystal Sugar Company
PO Box 1037
2500 North 11th Street
Moorhead, Minnesota 56560
January 8, 1990

Re: Bioaugmentation Treatability Studies
Sample #1 I.D.: Mud Pond Contents (M1289-146)
Sample #2 I.D.: Underflow (M1289-147)
Sample Date: December 18, 1989
Sample Received: December 19, 1989

BACKROUND

Following is the initial analysis of the samples received on December 19, 1989.

EXPERIMENTAL

Following this analysis, each of the two samples were divided into three equal portions, two of which were inoculated with AquaClean at concentrations of 10 and 25 PPM for the Mud Pond Contents and 25 and 50 PPM for the Underflow sample. The third portion of each sample was then utilized as a biological sample and was subjected to the same conditions throughout the Treatability study. The samples were monitored over a 12-day period, with the results as shown in Tables 1 and 2.

TABLE 1

Lab. No. M1289-146, Mud Pond Contents
(All Data is Reported in mg/l)

TABLE 2

Lab. No. M1289-147, Underflow
(All Data is Reported in mg/l

CONCLUSIONS

The effectiveness of the AquaClean upon the control of the parameters of interest is self-evident by comparing the results of the Treated portions of the sample with the results of the untreated portion of the sample.

In addition to the monitoring as shown in Table 1 and 2, a test called “The Odor Index” was also performed, utilizing a Head Space GC. The results of this testing are as follows:

Lab. No. M1289-146, Mud Pond

Lab. No. M1289-147, Underflow

The odor index is generally considered to measure odors from hydrogen sulfide content and odors produced by mercaptans and other materials that are often found in effluents from wastes similar to yours. The response as shown in the two portions of the sample which were treated with AquaClean reflect the potential for Aqua Clean to control odors in materials of this nature. I am not certain that complete control of all odors can be obtained at all times but, considerable reduction of the odor causing materials would result as a direct response to the use of AquaClean. By utilizing an initial seeding of AquaClean of approximately 5 to 7 PPM, based on the size of the lagoon, followed by a daily maintenance dose of 2 PPM based on flow into the system, adequate material populations cou1d be maintained which wou1d provide for the oxidation of the organic compounds and inorganic compounds, which are causing the emission of odor. You would be wise if treatment were commenced prior to the spring turnover, at least, as so far as the initial seeding of the lagoons are concerned. The daily maintenance once commenced, should continue throughout the year, as long as the lagoon was actively in use.

I hope this report is of use to you. If I can be of any further assistance or answer any further questions, please do not hesitate to call.