AquaClean Treatment & Benefits for Shrimp Farming

Application for a One Hectare / One Meter Deep Grow-Out Pond:

Second Week Fourth & Sixth Week Every Second Week

14 Gallons 7 / 3 Gallons 3 Gallons

Total Treatment is 45 Gallons for a Five Month Grow-Out Season….Benefits:

• Approximately 20 Percent increase in total yield…better yet, this is an

increase based on the individual growth, and therefore an increase in Grade.

• ProBiotic effect, shown in better health and

Feed Conversion improvement, with some 14 % less feed

• Significantly reduced sludge, lowering clean up costs in between seasons

• Improved water quality, meeting discharge regulations for BOD and Nutrients

• ACF-32 works in both Salt and Fresh Water Ponds

as used by Major Aquarium Suppliers worldwide

• The Feed Reduction Effect has been observed in both aquatic and non-aquatic university studies. Poultry Study in Syria showed improved growth and feed conversion by adding ACF-32 to the drinking water. Link is the similarity of poor digestive tracks in shrimp, fish, poultry and swine.

• Healthier Environment Effect is clearly seen in many of the ACF-32 applications, with significantly lower Sludge Handling costs, for shrimp allowing for faster turn-a-rounds in between seasons

• In addition…

BluePlanet can also treat White Spot andParasites…the issue is economic cost/benefit.

• Sabbactisun is an herbal oil product that aids in the recovery from white spot and bacterial infections.

• Parazoryne is an herbal formula that helps healing from parasitic infestations and infections.

• Sabbactisun and Parazoryne do not kill the nitrifying bacteria and do not reduce the water quality, which saves time and money. During application, the fish can be fed normally and water flow can remain in normal operation.

• TreatingWhite Spot with Sabbactisun…

• Application is based on the Pond’s Total Volume. Based on a One Hectare / One Meter Deep Pond, dosage is 20 to 25 Gallons per Day for 4 to 5 Days…

• The Cost/Benefit of treatment must be evaluated based on local market parameters, but based on saving a full season’s crop and stopping the potential spread to a second season is usually very economical.

• Parazoryne is applied at the same rate, but is less likely to be needed for shrimp farming issues.

Salt Water AquaCulture – Common Q&A for Shrimp Farmers

1) What is the different between Bacillus, Enzyme, and Aerobic Products and AquaClean?

The main difference between the ACF-32 (AquaClean and MICROBE-LIFT are identical products) and other bacterial and enzyme products is that the ACF-32 contains a broader spectrum of vegetative cells, i.e. cells that are viable, active cells whose activity is suppressed by the hydrogen sulfide in the product that acts as a metabolic inhibitor for the bacteria allowing them to survive in a bottle for two years. In much the same way an anesthetic puts a patient asleep for surgery, the hydrogen sulfide puts the bacteria into a type of anaesthetized state. It is still alive, but its metabolic rate is slowed down to almost nothing. Once the hydrogen sulfide flashes off and dilutes into the water, the bacteria "wake up" and become active again within minutes. Using this technology allows us to utilize many organisms that could not readily be preserved, limiting the genus and species from which selections could be made relative to metabolic capabilities.

Bacillus species are used in many products because they form spores that will maintain viability under storage for long periods of time. A spore is like a seed for a plant. It can resist dry conditions, heat, and many other circumstances that would adversely affect an organism. Once the spore is introduced into an environment suitable for growth, it germinates into a vegetative cell. This process may take several hours so it takes longer to see results from these products. The main limitation to spore-based products is they limit you to Bacillus and Clostridia genera. The Bacillus are primarily aerobic, while Clostridia are anaerobic.

Enzymes provide limited benefit since they are very specific in nature. The can help accelerate one rate limiting step, for example the use of a lipase to accelerate the solubilization of fats so they can be degraded faster. However, while the enzymes are catalysts that can catalyze reactions over and over, they are also protein molecules that are subject to chemical and biological breakdown, so their benefit is short lived. Also, since they are not living things, they do not reproduce like bacteria. This is a key factor that makes bacteria superior is that they are enzyme factories and will produce the wide array of enzymes required to catalyze many different biochemical reactions. In fact, bacteria adapt to their environment and will, within limited scope, product various types of enzymes able to feed upon the available and varied sources within a changing environment.

2) Why these competitive products do not work usefully in the seawater?

In order to main homeostasis, a bacteria functions best in what is called an iso-osmotic environment, where the salt concentration in the cell is similar to the environment in which it is living. When the salt concentration outside the cell is much higher than the salt concentration in the cell, water passively diffuses out of the cell to the external environment. This can result in dehydration of the cell and loss of viability. This is why honey does not spoil. The sugar concentration makes for a hyper-osmotic environment in which bacteria cannot grow.

In order to survive in a hyperosmotic environment, like salt water, the bacteria have to develop the ability to actively pump water back into the cell to maintain homeostasis, or the correct balance of water and electrolytes in the cell. Certain cells, like marine organisms, have the ability to do this effectively. This allows them to survive and dominate in marine environments. Many marine organisms have probably developed this capability over many generations of evolution. Through gradual adaptation, many non-marine organisms can be made to tolerate salt water environments as they establish these pumping mechanisms.

3) Why AquaClean can work in the sea water?

The organisms have shown the ability to adapt to the hyperosmotic environment in salt water as described above.

4) The best ways to increase DO value?

The best way to increase D.O. value is to increase aeration or go to a more efficient type of aeration and make sure that tanks are sized with the proper dimensions to take advantage of the capabilities of the aeration system, for example, to be deep enough to take maximum advantage of diffuser systems.

We have found that the photosynthetic bacteria in the ACF-32 relieve some of the load on the aerobes, breaking down waste organics with less oxygen. We have seen this lead to higher D.O. levels with no modifications to the aeration system or addition of oxygen release compounds (ORCs). Most solid peroxides that are used to add oxygen to the water tend to raise the pH a little too much to be used safely in aquaculture applications. ORC's are also an expensive way of adding oxygen relative to aeration systems. However, our OPC has been used in aquaculture systems safely to control algae and raise D.O. levels during short term drops in D.O. due to equipment failure, electrical failure, etc.

5) The best breeding methods of Shrimp against WSS (White Spot Disease)

As with any organism, providing a healthy environment that helps to maintain a higher level of resistance in the individual organisms is the best way to prevent diseases. The ACF-32 does this.

With many bacterial infections, the ACF-32 can reduce the incidence of these infections through competitive exclusion, as they compete with the bacterial pathogens for domination of the ecosystem. However, since White Spot Disease is caused by a virus, there is no control through competitive exclusion, at least that we know of or have documented.

Water control is very important for Fish and Shrimp Farmers because Whit Spot Disease comes from the polluted water. Therefore, AquaClean’s abilities to improve water quality; reducing Sludge, COD, BOD, Nitrogen Compounds and Ammonia, help create a healthier environment.

Many Shrimp Farmers are using bacillus subtillus products (10 ^11/g), but as you may know, it is not really useful. Bacillus is aerobic thermophile cultured over 45~60. By replacing these inferior products with AquaClean, the general condition of the Shrimp ponds will significantly improve…leading to greater yield.