Case Poultry Farm

AquaClean resulted in excellent bio-degradation of the BOD, COD and suspended solids, as well as qualitative reduction in odor from ammonia content in barns.

T.P. ASSOCIATES INTERNATIONAL, INC.
17875 Cherokee P.O. Box 96
Harpster, Ohio 43323
FAX # 614-496-4121
614-496-4571



Case Farm Poultry Processing
Qeuebrand Maintenance
Box 185
Winesburg, OH 44690
RE: Bio-Augmentation Treatability Studies
May 14, 1997

Background

Following are the results of the analysis we performed on samples received by us on April 27, 1997. The samples were identified as #1 Lagoon, #2 Lagoon and #3 Lagoon. These samples were analyzed initially to establish baseline data upon which treatment comparison can be made. This initial analysis also assures us that appropriate concentrations of nutrients were present for the growth of the microorganisms of Aqua Clean as well as to indicate if any inhibitory quantities of toxic materials were present. Following are the results of the initial analysis performed on these samples.

Experimental

Following this initial analysis, the #2 Lagoon sample was inoculated with bacteria at concentrations of 20 and 40 parts per million (PPM). These samples together with an untreated portion of the sample, which served as a control, were placed into a bio-oxidation system that simulates conditions found in your treatment lagoon. These samples were then monitored over a four day period. The following is a summary of the results of that monitoring.

Conclusiones

The #2 lagoon was the sample, which was selected for treatment due in, part to the highest BOD and COD values recorded. The sample showed excellent response to the bio-augmentation, especially in the 20 ppm level. The COD showed excellent reductions from the initial 9,231 mg/l to 333 mg/l on the monitoring date of May 1, 1997. The control sample on this date indicated a BOD of 667 mg/l and when the two are compared the results of the AquaClean can actually be seen. The suspended solids had dropped to 536 mg/l as compared to the control of 636 mg/l on May 4, 1997. The sulfides of the initial sample of 16.0 mg/l showed excellent response in the 40 ppm dropping to 3.2 mg/l over that period of time. Inhibiting factors must be considered such as the copper and zinc concentrations that were observed in this sample. A synergistic negative effect upon the lagoon operations is often seen as a result of these two metals being in concentrations of this nature. The threshold level of copper that can be tolerated prior to inhibiting characteristics being influenced upon bio-degradation is 500 ug/l (micrograms). The copper concentration of this sample was 677 ug/l and when viewed together with the 2,483 ug/l of zinc would offer an inhibiting concentration of these two metals resulting in low toxicity. As a result of this none of the normal biology, which would reduce ammonias and BOD, would be as active on these lagoons.

The addition of AquaClean has resulted in excellent bio- degradation of the BOD, COD and suspended solids. Although monitoring was not done on the ammonia content it was extrapolated that this compound would also undergo excellent bio-degradation by the use of AquaClean.

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